Russian is the primary language of instruction. All language, literature, and courses are taught in Russian.

Courses in history, political science, and area studies will be also taught in Russian. Professors will adjust the language to help students follow the explanations. English presentations and materials may be used.

Natural Sciences/STEM instructors are English- and Russian-speaking Daugavpils University professors. The language of instruction varies from English-only to English/Russian mix and up to Russian-only, depending on the instructor and the course.